Journal: Antioxidants

Article Title: DNA Damage in Human Amniotic Cells: Antigenotoxic Potential of Curcumin and α -Lipoic Acid

doi: 10.3390/antiox10071137

Figure Lengend Snippet: ( a ) Percentage of DNA fragmentation (ordinate) in amniotic cells after different hours of exposure (abscissa) to H 2 O 2 , α-lipoic acid (LA) and H 2 O 2 and LA combined. The black bars are negative control (NC); the white bars are positive control (10 mM H 2 O 2 ); the dark grey bars are 200 µM α-lipoic acid (LA 1 ); the light grey bars are 300 µM α-lipoic acid (LA 2 ); the striped bars are 10 mM H 2 O 2 + 200 µM α-lipoic acid co-treatment (LA 1 + H 2 O 2 ); the dotted bars are 10 mM H 2 O 2 + 300 µM α-lipoic acid co-treatment (LA 2 + H 2 O 2 ). ( b ) Percentage of DNA fragmentation (ordinate) in amniotic cells after different hours of exposure (abscissa) to H 2 O 2 , curcumin (Cur) and H 2 O 2 and Cur combined. The black bars are negative control (NC); the white bars are positive control (10 mM H 2 O 2 ); the dark grey bars are 20 µM curcumin (Cur 1 ); the light grey bars are 40 µM curcumin (Cur 2 ); the striped bars are 10 mM H 2 O 2 + 20 µM curcumin co-treatment (Cur 1 + H 2 O 2 ); the dotted bars are 10 mM H 2 O 2 + 40 µM curcumin co-treatment (Cur 2 + H 2 O 2 ). DNA fragmentation index was calculated as follows: DFI = (number of green nuclei/number of total nuclei) × 100). Values with different letters differ with statistical significance (ANOVA). p ≤ 0.05.

Article Snippet: Differences in the percentages of cell viability, DNA fragmentation and genomic stability among the experimental groups were analysed by applying ANOVA (analysis of variance) tests with GraphPad Prism 6.

Techniques: Negative Control, Positive Control

Journal: Antioxidants

Article Title: DNA Damage in Human Amniotic Cells: Antigenotoxic Potential of Curcumin and α -Lipoic Acid

doi: 10.3390/antiox10071137

Figure Lengend Snippet: ( a ) Percentage of DNA fragmentation (ordinate) in amniotic cells after different hours of exposure (abscissa) to H 2 O 2 , H 2 O 2 and LA (co-exposure), H 2 O 2 and curcumin (Cur) (co-exposure) and LA plus Cur and H 2 O 2 . The black bars are negative control (NC); the white bars are positive control (10 mM H 2 O 2 ); the light grey bars are 10 mM H 2 O 2 + 200 µM α-lipoic acid (LA 1 + H 2 O 2 ); the dark grey bars are 10 mM H 2 O 2 + 40 µM curcumin (Cur 2 + H 2 O 2 ); the grey dotted bars are 200 µM α-lipoic acid plus 40 µM curcumin with 10 mM H 2 O 2 co-treatment (LA 1 + Cur 2 + H 2 O 2 ). DNA fragmentation index was calculated as follows: DFI = (number of green nuclei/number of total nuclei) × 100). Values with different letters differ with statistical significance (ANOVA). p ≤ 0.05. ( b ) Amniotic cells with fragmented DNA (green) and intact DNA (blue), after H 2 O 2 treatment, detected with Nikon E600 fluorescence microscope.

Article Snippet: Differences in the percentages of cell viability, DNA fragmentation and genomic stability among the experimental groups were analysed by applying ANOVA (analysis of variance) tests with GraphPad Prism 6.

Techniques: Negative Control, Positive Control, Fluorescence, Microscopy